The Rat BNP (Brain Natriuretic Peptide) ELISA Kit is an advanced assay meticulously developed for the precise quantitative determination of Brain Natriuretic Peptide levels in diverse biological samples obtained from rat subjects.
Brain Natriuretic Peptide, a crucial marker of cardiac health, serves as a profound indicator of cardiac stress and heart failure. As a multifaceted peptide hormone, BNP plays a pivotal role in regulating blood pressure, fluid balance, and cardiovascular response, making it a vital target for cardiovascular research, diagnostic evaluations, and therapeutic assessments. This state-of-the-art ELISA kit from Assay Genie boasts exceptional sensitivity and specificity, ensuring accurate and reproducible results. Manufactured under stringent quality control standards, this kit delivers robust performance, facilitating precise measurements of BNP levels with ease and reliability. Researchers can rely on this ELISA kit to unravel the intricate dynamics of Brain Natriuretic Peptide in cardiovascular pathophysiology and therapeutic interventions, making it an indispensable tool for comprehensive cardiovascular studies.
Product Name:
Rat BNP (Brain Natriuretic Peptide) ELISA Kit
SKU:
AEES00332
Target:
Rat BNP (Brain Natriuretic Peptide)
Size:
96T
Assay type:
Competitive-ELISA
Assay time:
2.0h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with BNP. During the reaction, BNP in the sample or standard competes with a fixed amount of BNP on the solid phase supporter for sites on the Biotinylated Detection Ab specific to BNP. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of BNP in tested samples can be calculated by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
90-101
98-113
97-112
Average (%)
96
105
105
1:4
Range (%)
83-95
89-102
95-111
Average (%)
90
96
101
1:8
Range (%)
88-103
89-106
100-111
Average (%)
95
97
105
1:16
Range (%)
83-94
88-101
100-114
Average (%)
89
94
107
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
93-107
101
EDTA plasma (n=5)
84-97
91
Cell culture media (n=5)
86-98
92
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
96.2
313.1
672.8
97.2
338.8
634.5
Standard deviation
5.9
14.7
32.3
6.6
17.3
29.2
C V (%)
6.13
4.69
4.8
6.79
5.11
4.6
Sample type &Sample volume:
serum, plasma and other biological fluids; 50μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of BNP concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes BNP in samples. No significant cross-reactivity or interference between BNP and analogues was observed.
