Rat Brain Derived Neurotrophic Factor (BDNF) ELISA Kit
The Rat Brain-Derived Neurotrophic Factor (BDNF) ELISA Kit is specifically designed for precise and reliable detection of BDNF levels in rat brain samples. This kit offers exceptional sensitivity and specificity, allowing for accurate and reproducible results that are essential for various research applications.BDNF is a key neurotrophin that plays a vital role in neuronal survival, growth, and differentiation. It is crucial for maintaining brain function and plasticity, making it a valuable marker for studying neurological disorders, such as Alzheimer's disease, depression, and schizophrenia.
By measuring BDNF levels, researchers can gain valuable insights into the mechanisms underlying these conditions and facilitate the development of potential therapeutic interventions.Overall, the Rat Brain-Derived Neurotrophic Factor (BDNF) ELISA Kit is an indispensable tool for researchers studying neurological disorders and neurotrophic factors in rat models, providing accurate and reliable data for cutting-edge research in the field of neuroscience.
Product Name:
Rat Brain Derived Neurotrophic Factor (BDNF) ELISA Kit
Product Code:
RTDL00138
Size:
96 Assays
Target:
BDNF
Synonyms:
Neurotrophin, Abrineurin
Detection Method:
Sandwich
Range:
0.156-10ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
