The Rat C1 Complement Component 1 ELISA Kit is specifically designed for the precise measurement of C1 complement component 1 levels in rat samples. This kit offers exceptional sensitivity and specificity, guaranteeing accurate and consistent results, which makes it perfect for various research applications.C1 complement component 1 is an essential protein involved in the complement system, playing a crucial role in immune response and inflammation.
Abnormal levels of C1 complement component 1 have been associated with various autoimmune diseases, infections, and inflammatory conditions, making it a valuable biomarker for understanding these diseases and developing effective treatments. Overall, the Rat C1 Complement Component 1 ELISA Kit is a reliable tool for researchers looking to investigate the role of C1 complement component 1 in various physiological and pathological processes in rats.
Product Name:
Rat C1 (Complement Component 1) ELISA Kit
SKU:
RTES00919
Target:
Rat C1 (Complement Component 1)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.47 ng/mL
Detection range:
0.78-50 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat C1q. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat C1q and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat C1q, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat C1q. You can calculate the concentration of Rat C1q in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
94-109
89-105
97-111
Average (%)
102
96
102
1:4
Range (%)
88-100
85-99
85-98
Average (%)
94
90
90
1:8
Range (%)
86-102
86-101
85-96
Average (%)
93
93
90
1:16
Range (%)
90-103
84-99
85-95
Average (%)
98
91
90
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
95-106
100
EDTA plasma (n=5)
88-100
93
Cell culture media (n=5)
90-102
96
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
2.58
6.64
17.88
2.83
6.88
16.82
Standard deviation
0.15
0.33
0.66
0.19
0.36
0.63
C V (%)
5.81
4.97
3.69
6.71
5.23
3.75
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Rat C1q concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat C1q in samples. No significant cross-reactivity or interference between Rat C1q and analogues was observed.
