The Rat CAM (Calmodulin) CLIA Kit is specifically designed for the quantitative measurement of calmodulin levels in rat samples, including serum, plasma, and tissue homogenates. This kit offers high sensitivity and accuracy, ensuring precise and reliable results for a variety of research applications.Calmodulin is a vital calcium-binding protein that plays a key role in regulating various cellular processes, including muscle contraction, neurotransmitter release, and gene expression.
Dysregulation of calmodulin has been implicated in various diseases, making it an important biomarker for studying the mechanisms underlying these conditions and developing targeted therapies.Overall, the Rat CAM (Calmodulin) CLIA Kit provides researchers with a valuable tool for investigating the role of calmodulin in physiological and pathological processes in rats, ultimately contributing to advancements in biomedical research and potential clinical applications.
Product Name:
Rat CAM (Calmodulin) CLIA Kit
SKU:
RTES00081
Target:
Rat CAM (Calmodulin)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat CAM. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat CAM and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat CAM, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat CAM. You can calculate the concentration of Rat CAM in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
84-97
92-105
100-116
Average (%)
91
98
107
1:4
Range (%)
87-99
99-113
98-111
Average (%)
93
105
104
1:8
Range (%)
89-101
90-103
94-108
Average (%)
95
95
100
1:16
Range (%)
98-110
96-111
86-98
Average (%)
104
104
93
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
99-112
106
EDTA plasma (n=5)
91-106
98
Cell culture media (n=5)
97-115
105
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
107.93
209.92
868.71
114.21
215.59
825.37
Standard deviation
10.2
23.83
87.83
12.36
24.28
62.48
C V (%)
9.45
11.35
10.11
10.82
11.26
7.57
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat CAM concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat CAM in samples. No significant cross-reactivity or interference between Rat CAM and analogues was observed.
