The Rat CK-MB (Creatine Kinase-MB Isoenzyme) CLIA Kit is specifically designed for the precise measurement of CK-MB levels in rat serum, plasma, and tissue homogenates. This kit offers unparalleled sensitivity and accuracy, ensuring dependable and consistent results for a variety of research purposes.CK-MB is a cardiac-specific biomarker that is released into the bloodstream following a heart attack or other cardiac conditions.
Monitoring CK-MB levels can provide valuable insights into cardiac health and help diagnose and manage cardiovascular diseases. This kit is an invaluable tool for studying cardiac biomarkers and advancing research in the field of cardiovascular medicine.
Product Name:
Rat CK-MB (Creatine Kinase MB Isoenzyme) CLIA Kit
SKU:
RTES00620
Target:
Rat CK-MB (Creatine Kinase MB Isoenzyme)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
7.50 pg/mL
Detection range:
12.50-800 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat CK-MB. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat CK-MB and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat CK-MB, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat CK-MB. You can calculate the concentration of Rat CK-MB in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
98-112
96-108
90-103
Average (%)
106
103
97
1:4
Range (%)
92-106
100-115
85-97
Average (%)
98
107
91
1:8
Range (%)
90-106
93-105
100-114
Average (%)
97
99
107
1:16
Range (%)
103-117
91-103
86-99
Average (%)
109
97
91
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
97-110
104
EDTA plasma (n=5)
92-105
99
Cell culture media (n=5)
89-101
94
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
43.11
127.93
343.8
44.97
139.7
357.57
Standard deviation
3.72
13.65
38.57
4.58
10.24
37.51
C V (%)
8.63
10.67
11.22
10.18
7.33
10.49
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat CK-MB concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat CK-MB in samples. No significant cross-reactivity or interference between Rat CK-MB and analogues was observed.
