Rat CK-MB (Creatine Kinase MB Isoenzyme) ELISA Kit
The Rat CK-MB (Creatine Kinase-MB Isoenzyme) ELISA Kit is a reliable and accurate tool for detecting levels of CK-MB in rat samples such as serum, plasma, and cell culture supernatants. With high sensitivity and specificity, this kit provides precise and reproducible results, making it suitable for various research applications.CK-MB is an important enzyme that plays a key role in cardiac muscle health, particularly in diagnosing and monitoring myocardial infarction (heart attack).
By measuring CK-MB levels, researchers and healthcare professionals can assess heart damage and track recovery progress in animal models, offering valuable insights for cardiovascular research and drug development efforts.
Product Name:
Rat CK-MB (Creatine Kinase MB Isoenzyme) ELISA Kit
SKU:
RTES00976
Target:
Rat CK-MB (Creatine Kinase MB Isoenzyme)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat CKMB. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat CKMB and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat CKMB, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat CKMB. You can calculate the concentration of Rat CKMB in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
96-107
97-112
91-105
Average (%)
102
102
96
1:4
Range (%)
95-105
87-98
87-102
Average (%)
100
92
94
1:8
Range (%)
90-103
82-93
87-101
Average (%)
97
88
93
1:16
Range (%)
92-107
82-92
86-100
Average (%)
99
87
93
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
86-100
93
EDTA plasma (n=5)
90-105
98
Cell culture media (n=5)
87-99
93
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
93.35
286.64
760.84
100.16
266.78
728.82
Standard deviation
4.85
13.27
36.44
6.24
15.71
23.91
C V (%)
5.2
4.63
4.79
6.23
5.89
3.28
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Rat CKMB concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat CKMB in samples. No significant cross-reactivity or interference between Rat CKMB and analogues was observed.
