The Rat CNTF (Ciliary Neurotrophic Factor) ELISA Kit is a specialized assay designed for the quantitative measurement of CNTF levels in rat biological samples. CNTF is a neurotrophic factor known for its essential role in the survival and differentiation of neuronal cells in the peripheral and central nervous systems.
This ELISA kit allows researchers to accurately quantify CNTF levels, providing valuable insights into neurodevelopment, neural repair, and neuroprotection mechanisms. By detecting and measuring CNTF levels, researchers can gain a deeper understanding of its functions in neuronal growth, synaptic plasticity, and neuroprotection. CNTF has been implicated in various neurological conditions, making accurate measurement crucial for studying its involvement in neurodegenerative diseases, neural injury, and other nervous system disorders. The Rat CNTF ELISA Kit from Assay Genie offers exceptional sensitivity and specificity, ensuring precise and reproducible results. With stringent quality control standards in place, this kit delivers reliable performance and ease of use, making it a powerful tool for research focused on unraveling the neuroprotective and neuroregenerative properties of Ciliary Neurotrophic Factor.
Product Name:
Rat CNTF (Ciliary Neurotrophic Factor) ELISA Kit
SKU:
AEES00351
Target:
Rat CNTF (Ciliary Neurotrophic Factor)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
23.44 pg/mL
Detection range:
39.06-2500 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat CNTF. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat CNTF and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat CNTF, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat CNTF. You can calculate the concentration of Rat CNTF in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
99-113
93-106
87-100
Average (%)
106
98
93
1:4
Range (%)
90-102
80-94
83-94
Average (%)
97
86
89
1:8
Range (%)
89-104
82-93
89-102
Average (%)
96
88
95
1:16
Range (%)
89-99
81-92
86-98
Average (%)
94
86
91
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
88-102
94
EDTA plasma (n=5)
96-109
102
Cell culture media (n=5)
91-105
99
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
115.36
349.14
1116.2
125.92
347.38
1163.07
Standard deviation
6.31
15.19
44.2
8.78
14.1
48.27
C V (%)
5.47
4.35
3.96
6.97
4.06
4.15
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Rat CNTF concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat CNTF in samples. No significant cross-reactivity or interference between Rat CNTF and analogues was observed.
