The Rat CNTN1 (Contactin-1) CLIA Kit is a highly sensitive and specific assay designed for the accurate detection of contactin-1 levels in rat serum, plasma, and cell culture supernatants. Contactin-1 is a key protein involved in cell adhesion and signal transduction processes, playing a critical role in nervous system development and function.This cutting-edge kit provides reliable and reproducible results, making it an ideal tool for a wide range of research applications in neuroscience, developmental biology, and neurology.
By accurately quantifying contactin-1 levels, researchers can gain valuable insights into the mechanisms underlying neurological disorders, such as multiple sclerosis and autism, as well as potential therapeutic targets for intervention.Trust the Rat CNTN1 CLIA Kit from Assay Genie to enhance your research and uncover new discoveries in the field of neuroscience.
Product Name:
Rat CNTN1 (Contactin 1) CLIA Kit
SKU:
RTES00152
Target:
Rat CNTN1 (Contactin 1)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat CNTN1. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat CNTN1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat CNTN1, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat CNTN1. You can calculate the concentration of Rat CNTN1 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
101-113
91-103
102-116
Average (%)
107
97
110
1:4
Range (%)
97-110
90-103
97-114
Average (%)
102
97
105
1:8
Range (%)
95-107
103-115
96-109
Average (%)
100
109
104
1:16
Range (%)
91-103
87-101
94-109
Average (%)
96
93
101
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
89-101
96
EDTA plasma (n=5)
99-114
106
Cell culture media (n=5)
93-110
101
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
97.04
210.27
937.92
106.63
214.0
852.37
Standard deviation
8.18
22.35
80.75
11.56
19.84
77.31
C V (%)
8.43
10.63
8.61
10.84
9.27
9.07
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat CNTN1 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat CNTN1 in samples. No significant cross-reactivity or interference between Rat CNTN1 and analogues was observed.
