The Rat COL2 (Collagen Type II) ELISA Kit is a state-of-the-art assay designed for the precise quantitative measurement of Collagen Type II levels in a variety of rat biological samples. Collagen Type II is a critical structural protein that forms the matrix of cartilage tissues in the body, providing essential support and resilience to cartilaginous structures. This ELISA kit offers researchers a reliable tool to quantify the concentration of Collagen Type II, enabling a deeper understanding of cartilage biology, joint health, and musculoskeletal disorders. Accurate quantification of Collagen Type II levels is crucial for elucidating its role in cartilage development, maintenance, and pathologies such as osteoarthritis.
With the Rat COL2 ELISA Kit, researchers can investigate the dynamics of Collagen Type II expression, explore its involvement in cartilage homeostasis, and potentially identify therapeutic strategies for cartilage-related conditions. Powered by highly sensitive and specific detection mechanisms, Assay Genie's Rat COL2 ELISA Kit ensures trustworthy and reproducible results. Manufactured under strict quality control standards, this kit delivers robust performance and user-friendly protocols, making it an excellent choice for research studies exploring Collagen Type II biology in the context of skeletal tissues and cartilage function.
Product Name:
Rat COL2 (Collagen Type II) ELISA Kit
SKU:
AEES00359
Target:
Rat COL2 (Collagen Type Ⅱ)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
37.50 pg/mL
Detection range:
62.50-4000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat COL2. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat COL2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat COL2, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat COL2. You can calculate the concentration of Rat COL2 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
99-111
87-101
97-114
Average (%)
105
92
104
1:4
Range (%)
90-104
83-95
88-102
Average (%)
96
90
94
1:8
Range (%)
92-108
83-97
85-98
Average (%)
99
90
90
1:16
Range (%)
87-102
82-94
83-95
Average (%)
93
87
90
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
89-100
94
EDTA plasma (n=5)
93-106
99
Cell culture media (n=5)
94-109
101
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
190.88
496.47
1366.4
207.53
453.36
1285.96
Standard deviation
12.66
29.44
57.39
12.76
19.04
64.17
C V (%)
6.63
5.93
4.2
6.15
4.2
4.99
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Rat COL2 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat COL2 in samples. No significant cross-reactivity or interference between Rat COL2 and analogues was observed.
