Rat COL4 alpha1 (Collagen Type IV Alpha 1) CLIA Kit
The Rat COL4 alpha1 (Collagen Type IV Alpha 1) CLIA Kit is a specialized assay kit designed for the accurate measurement of COL4 alpha1 levels in rat samples. This kit offers high sensitivity and specificity, allowing for reliable and reproducible results in a variety of research applications.COL4 alpha1 is a key component of collagen type IV, an essential protein in the extracellular matrix that provides structural support to tissues and organs. Abnormalities in COL4 alpha1 levels have been associated with various disease conditions, including kidney diseases, muscular dystrophies, and vascular disorders.
Therefore, this CLIA kit serves as a valuable tool for studying these conditions and exploring potential therapeutic interventions.Overall, the Rat COL4 alpha1 CLIA Kit from AssayGenie is a valuable resource for researchers looking to accurately quantify COL4 alpha1 levels in rat samples, advancing our understanding of collagen biology and its implications in disease pathology.
Product Name:
Rat COL4 alpha1 (Collagen Type IV Alpha 1) CLIA Kit
SKU:
RTES00141
Target:
Rat COL4 alpha1 (Collagen Type IV Alpha 1)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
0.19 ng/mL
Detection range:
0.31-20 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat COL4α1. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat COL4α1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat COL4α1, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat COL4α1. You can calculate the concentration of Rat COL4α1 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
94-107
98-116
102-117
Average (%)
100
106
108
1:4
Range (%)
87-100
85-97
92-107
Average (%)
94
91
99
1:8
Range (%)
100-118
101-114
92-109
Average (%)
108
107
99
1:16
Range (%)
96-110
93-107
90-102
Average (%)
102
100
96
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
87-100
94
EDTA plasma (n=5)
92-109
100
Cell culture media (n=5)
88-101
93
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
1.07
3.2
9.74
1.1
3.18
10.71
Standard deviation
0.12
0.26
1.07
0.11
0.23
0.85
C V (%)
11.21
8.13
10.99
10.0
7.23
7.94
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat COL4α1 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat COL4α1 in samples. No significant cross-reactivity or interference between Rat COL4α1 and analogues was observed.
