Rat COMP (Cartilage Oligomeric Matrix Protein) CLIA Kit
The Rat COMP (Cartilage Oligomeric Matrix Protein) CLIA Kit is specifically designed for the quantitative measurement of COMP levels in rat serum, plasma, and tissue homogenates. This kit offers high sensitivity and specificity, ensuring accurate and reliable results for various research applications.COMP is a key protein found in cartilage tissues and plays a crucial role in maintaining cartilage integrity and function.
Changes in COMP levels have been associated with various joint diseases, including osteoarthritis and rheumatoid arthritis. Therefore, the Rat COMP CLIA Kit serves as a valuable tool for studying cartilage-related disorders and exploring potential treatment options.
Product Name:
Rat COMP (Cartilage Oligomeric Matrix Protein) CLIA Kit
SKU:
RTES00095
Target:
Rat COMP (Cartilage Oligomeric Matrix Protein)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
0.19 ng/mL
Detection range:
0.31-20 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat COMP. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat COMP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat COMP, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat COMP. You can calculate the concentration of Rat COMP in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
89-102
103-121
90-103
Average (%)
96
110
96
1:4
Range (%)
97-114
92-108
93-107
Average (%)
104
99
100
1:8
Range (%)
89-100
91-107
99-113
Average (%)
95
97
106
1:16
Range (%)
99-117
101-114
96-111
Average (%)
108
107
102
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
99-113
105
EDTA plasma (n=5)
97-114
104
Cell culture media (n=5)
97-110
104
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
1.04
2.39
7.47
1.0
2.31
8.0
Standard deviation
0.11
0.29
0.57
0.12
0.24
0.53
C V (%)
10.58
12.13
7.63
12.0
10.39
6.63
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat COMP concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat COMP in samples. No significant cross-reactivity or interference between Rat COMP and analogues was observed.
