Rat CRH (Corticotropin Releasing Hormone) ELISA Kit
The Rat CRH (Corticotropin Releasing Hormone) ELISA Kit is a state-of-the-art assay system developed to precisely quantify levels of CRH in various rat biological samples. Corticotropin Releasing Hormone serves as a critical mediator of the body's stress response, controlling the release of hormones that affect functions such as metabolism, immune response, and behavior. By using this ELISA kit, researchers can accurately measure CRH levels, facilitating a deeper understanding of the body's stress regulation mechanisms and their impact on physiological processes and adaptations. CRH plays a pivotal role in orchestrating the body's response to stress, influencing adrenal gland function and the production of stress-related hormones like cortisol.
Through the precise measurement of CRH using this ELISA kit, researchers can gain valuable insights into the intricate pathways involved in stress regulation, neuroendocrine responses, and the broader physiological effects triggered by stress-related stimuli. The Rat CRH (Corticotropin Releasing Hormone) ELISA Kit offered by Assay Genie delivers exceptional sensitivity and specificity, ensuring accurate and reproducible results. Manufactured under rigorous quality control standards, this kit provides robust performance and user-friendly protocols, making it an excellent choice for researchers investigating stress responses, neuroendocrine pathways, and related physiological processes.
Product Name:
Rat CRH (Corticotropin Releasing Hormone) ELISA Kit
SKU:
AEES00369
Target:
Rat CRH (Corticotropin Releasing Hormone)
Size:
96T
Assay type:
Competitive-ELISA
Assay time:
2.0h
Sensitivity:
0.19 ng/mL
Detection range:
0.31-20 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with CRH. During the reaction, CRH in the sample or standard competes with a fixed amount of CRH on the solid phase supporter for sites on the Biotinylated Detection Ab specific to CRH. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of CRH in tested samples can be calculated by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
86-98
95-112
98-115
Average (%)
92
102
105
1:4
Range (%)
89-100
89-103
94-110
Average (%)
94
94
100
1:8
Range (%)
88-101
87-99
94-109
Average (%)
94
92
100
1:16
Range (%)
84-98
92-107
99-117
Average (%)
89
100
107
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
84-99
91
EDTA plasma (n=5)
86-98
91
Cell culture media (n=5)
89-99
94
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
1
2.7
8.9
1.1
2.7
8.2
Standard deviation
0.1
0.1
0.4
0.1
0.1
0.4
C V (%)
10
3.7
4.49
9.09
3.7
4.88
Sample type &Sample volume:
serum, plasma and other biological fluids; 50μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of CRH concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes CRH in samples. No significant cross-reactivity or interference between CRH and analogues was observed.
