The Rat Calcitonin (CT) ELISA Kit is a cutting-edge assay meticulously designed for the precise and quantitative measurement of Calcitonin levels in various rat biological samples.
Calcitonin, a vital hormone produced by the thyroid, is integral in regulating calcium and phosphorus metabolism, making it essential for bone health and calcium balance in the body. Given its role in modulating calcium levels, Calcitonin is crucial for maintaining bone mineral density and homeostasis. This ELISA kit offers researchers a reliable tool to explore the dynamic changes in Calcitonin levels, shedding light on its involvement in skeletal health, calcium regulation, and potential therapeutic applications. With exceptional sensitivity and specificity, this assay ensures accurate and reproducible results, making it an invaluable asset for investigations into Calcitonin biology in physiological and pathological conditions. Manufactured under stringent quality control standards, this kit guarantees robust performance, providing researchers with a user-friendly and efficient solution for scientific exploration and discovery.
Product Name:
Rat CT (Calcitonin) ELISA Kit
SKU:
AEES00307
Target:
Rat CT (Calcitonin)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat CT. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat CT and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat CT, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat CT. You can calculate the concentration of Rat CT in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
92-110
90-104
98-110
Average (%)
100
97
104
1:4
Range (%)
98-116
86-100
90-104
Average (%)
106
92
97
1:8
Range (%)
101-116
88-99
90-105
Average (%)
107
93
98
1:16
Range (%)
95-109
85-98
99-114
Average (%)
101
92
105
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
92-105
99
EDTA plasma (n=5)
86-98
93
Cell culture media (n=5)
91-102
96
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
46.6
124.2
434.2
49.8
113.7
400.0
Standard deviation
2.7
6.1
19.1
3.0
5.2
14.8
C V (%)
5.79
4.91
4.4
6.02
4.57
3.7
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Rat CT concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat CT in samples. No significant cross-reactivity or interference between Rat CT and analogues was observed.
