Rat cTnT/TNNT2 (Troponin T Type 2, Cardiac) CLIA Kit
The Rat CTnT (TNNT2) Troponin T Type 2 Cardiac CLIA Kit is specifically designed for the quantitative measurement of Troponin T (cTnT), a cardiac biomarker, in rat serum, plasma, and tissue homogenates. This kit offers high sensitivity and specificity, ensuring accurate and reproducible results for researchers studying cardiac health and disease.Troponin T is a key protein involved in cardiac muscle contraction and is released into the bloodstream following heart damage or injury. Elevated levels of cTnT are indicative of cardiac injury, making it a vital marker for diagnosing and monitoring conditions such as myocardial infarction and heart failure.
By using the Rat CTnT (TNNT2) Troponin T Type 2 Cardiac CLIA Kit, researchers can gain valuable insights into cardiac function, assess the extent of cardiac damage, and evaluate the efficacy of potential treatments. This kit is an essential tool for advancing our understanding of cardiac pathology and developing novel therapeutic strategies for cardiovascular diseases in rat models.
Product Name:
Rat cTnT/TNNT2 (Troponin T Type 2, Cardiac) CLIA Kit
SKU:
RTES00089
Target:
Rat cTnT/TNNT2 (Troponin T Type 2, Cardiac)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
4.69 pg/mL
Detection range:
7.81-500 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat cTnT/TNNT2. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat cTnT/TNNT2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat cTnT/TNNT2, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat cTnT/TNNT2. You can calculate the concentration of Rat cTnT/TNNT2 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
86-96
103-121
100-113
Average (%)
91
110
105
1:4
Range (%)
85-98
93-103
84-96
Average (%)
91
98
90
1:8
Range (%)
104-119
97-108
87-101
Average (%)
110
103
93
1:16
Range (%)
84-95
97-113
89-101
Average (%)
90
103
94
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
87-100
93
EDTA plasma (n=5)
87-102
94
Cell culture media (n=5)
93-108
101
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
26.96
78.6
169.72
25.65
74.15
154.43
Standard deviation
2.99
7.05
16.41
2.71
5.4
13.84
C V (%)
11.09
8.97
9.67
10.57
7.28
8.96
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat cTnT/TNNT2 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat cTnT/TNNT2 in samples. No significant cross-reactivity or interference between Rat cTnT/TNNT2 and analogues was observed.
