The Rat Cys-C (Cystatin C) CLIA Kit is specifically designed for the quantitative detection of Cystatin C levels in rat serum, plasma, and cell culture supernatants. This kit offers a high level of sensitivity and specificity, providing accurate and reliable results for various research applications. Cystatin C is a vital protein involved in the regulation of cysteine proteases and plays a crucial role in the maintenance of normal physiological functions. Abnormal levels of Cystatin C have been associated with various diseases and conditions, including kidney dysfunction, cardiovascular diseases, and neurodegenerative disorders.
By accurately measuring Cystatin C levels, researchers can better understand the pathophysiology of these conditions and explore potential therapeutic interventions. The Rat Cys-C CLIA Kit provides a valuable tool for studying the role of Cystatin C in health and disease, offering researchers a reliable and efficient method for biomarker analysis.
Product Name:
Rat Cys-C (Cystatin C) CLIA Kit
SKU:
RTES00167
Target:
Rat Cys-C (Cystatin C)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat Cys-C. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat Cys-C and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat Cys-C, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat Cys-C. You can calculate the concentration of Rat Cys-C in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
88-102
104-118
90-101
Average (%)
93
110
95
1:4
Range (%)
93-107
85-99
91-104
Average (%)
101
91
99
1:8
Range (%)
99-117
90-105
103-120
Average (%)
107
98
109
1:16
Range (%)
89-105
91-107
98-109
Average (%)
96
97
104
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
97-111
104
EDTA plasma (n=5)
88-99
94
Cell culture media (n=5)
100-116
108
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
96.99
311.68
829.38
100.67
280.87
749.94
Standard deviation
11.66
31.6
62.78
10.89
20.31
57.6
C V (%)
12.02
10.14
7.57
10.82
7.23
7.68
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat Cys-C concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat Cys-C in samples. No significant cross-reactivity or interference between Rat Cys-C and analogues was observed.
