The Rat Cyt C (Cytochrome C) CLIA Kit is specifically designed for the reliable and accurate measurement of cytochrome C levels in rat samples, including serum, plasma, and tissue homogenates. This kit offers high sensitivity and specificity, ensuring precise and reproducible results for various research applications.Cytochrome C is a key protein in the electron transport chain, playing a crucial role in cellular respiration and apoptosis. Abnormal levels of cytochrome C have been associated with various diseases, including cancer, neurodegenerative disorders, and cardiovascular diseases, making it a valuable biomarker for studying these conditions and developing potential therapeutic interventions.
Overall, the Rat Cyt C (Cytochrome C) CLIA Kit provides researchers with a powerful tool for investigating the role of cytochrome C in health and disease, ultimately advancing our understanding of cellular processes and potential treatment options.
Product Name:
Rat Cyt-C (Cytochrome C) CLIA Kit
SKU:
RTES00006
Target:
Rat Cyt-C (Cytochrome C)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
46.88 pg/mL
Detection range:
78.13-5000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat Cyt-C. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat Cyt-C and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat Cyt-C, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat Cyt-C. You can calculate the concentration of Rat Cyt-C in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
104-117
99-114
90-105
Average (%)
110
107
96
1:4
Range (%)
89-104
98-112
91-103
Average (%)
95
103
96
1:8
Range (%)
102-118
95-112
101-113
Average (%)
108
102
107
1:16
Range (%)
101-115
92-105
102-116
Average (%)
109
98
108
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
93-108
98
EDTA plasma (n=5)
87-99
92
Cell culture media (n=5)
88-105
96
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
257.49
689.88
1774.8
266.14
739.71
1631.97
Standard deviation
24.38
70.85
141.63
32.34
70.57
141.17
C V (%)
9.47
10.27
7.98
12.15
9.54
8.65
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat Cyt-C concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat Cyt-C in samples. No significant cross-reactivity or interference between Rat Cyt-C and analogues was observed.
