The Rat Cyt-C (Cytochrome C) ELISA Kit is a specialized assay designed for the quantitative detection of Cytochrome C levels in rat biological samples.
Cytochrome C is a crucial component of the electron transport chain involved in cellular respiration and apoptosis. This ELISA kit offers exceptional sensitivity and specificity, enabling accurate measurement of Cytochrome C concentrations in rat samples. Cytochrome C plays a key role in mitochondrial function and regulation, making it a valuable biomarker for studying mitochondrial dysfunction, oxidative stress, and apoptotic pathways. Manufactured under stringent quality control standards, this ELISA kit ensures robust performance, reliable results, and ease of use, making it an excellent choice for researchers investigating the roles of Cytochrome C in cellular processes and disease mechanisms.
Product Name:
Rat Cyt-C (Cytochrome C) ELISA Kit
SKU:
AEES00289
Target:
Rat Cyt-C (Cytochrome C)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.23 ng/mL
Detection range:
0.39-25 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat Cyt-C. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat Cyt-C and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat Cyt-C, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat Cyt-C. You can calculate the concentration of Rat Cyt-C in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
97-112
86-101
91-106
Average (%)
103
93
98
1:4
Range (%)
89-103
82-97
88-101
Average (%)
96
89
94
1:8
Range (%)
88-103
81-93
88-100
Average (%)
94
87
93
1:16
Range (%)
88-103
83-98
83-98
Average (%)
94
89
89
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
90-105
97
EDTA plasma (n=5)
86-97
91
Cell culture media (n=5)
96-107
102
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
1.33
4.0
8.84
1.36
3.69
8.71
Standard deviation
0.08
0.16
0.28
0.09
0.17
0.29
C V (%)
6.02
4.0
3.17
6.62
4.61
3.33
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Rat Cyt-C concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat Cyt-C in samples. No significant cross-reactivity or interference between Rat Cyt-C and analogues was observed.
