The Rat DAO (Diamine Oxidase) CLIA Kit is a reliable and accurate tool for detecting levels of diamine oxidase in rat serum, plasma, and tissue extracts. This kit offers high sensitivity and specificity, ensuring precise and reproducible results for various research applications.Diamine oxidase is an important enzyme involved in the metabolism of histamine and other biogenic amines, playing a vital role in the regulation of inflammation and allergic reactions. Abnormal levels of DAO have been associated with conditions such as food intolerances, histamine intolerance, and gastrointestinal disorders, making it a valuable biomarker for studying these diseases and exploring potential therapeutic interventions.
Overall, the Rat DAO CLIA Kit provides researchers with a powerful tool for investigating the role of diamine oxidase in health and disease, offering a comprehensive and reliable solution for measuring DAO levels in rat samples.
Product Name:
Rat DAO (Diamine Oxidase) CLIA Kit
SKU:
RTES00179
Target:
Rat DAO (Diamine Oxidase)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
0.38 ng/mL
Detection range:
0.63-40 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat DAO. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat DAO and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat DAO, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat DAO. You can calculate the concentration of Rat DAO in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
95-107
101-116
88-102
Average (%)
101
109
95
1:4
Range (%)
100-113
87-101
93-107
Average (%)
107
94
99
1:8
Range (%)
96-109
99-117
97-114
Average (%)
102
107
105
1:16
Range (%)
90-104
98-113
95-109
Average (%)
95
103
102
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
86-100
93
EDTA plasma (n=5)
90-105
97
Cell culture media (n=5)
90-101
96
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
1.9
4.82
13.33
2.03
5.16
14.56
Standard deviation
0.25
0.45
0.99
0.2
0.59
1.28
C V (%)
13.16
9.34
7.43
9.85
11.43
8.79
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat DAO concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat DAO in samples. No significant cross-reactivity or interference between Rat DAO and analogues was observed.
