The Rat DAT (Dopamine Transporter) CLIA Kit is specifically designed for the quantitative measurement of dopamine transporter levels in rat samples. This kit offers high sensitivity and specificity, ensuring accurate and reliable results for research applications involving dopamine transporter function.The dopamine transporter is a key protein involved in the regulation of dopamine levels in the brain, impacting various neurological processes including reward, motor control, and mood. Dysregulation of the dopamine transporter has been implicated in conditions such as Parkinson's disease, substance abuse disorders, and psychiatric illnesses, highlighting its importance as a biomarker for studying these disorders and potential therapeutic interventions.
Overall, the Rat DAT CLIA Kit provides researchers with a valuable tool for investigating the role of the dopamine transporter in physiological and pathological conditions in rats, ultimately contributing to a better understanding of dopamine-related mechanisms and potential treatment strategies.
Product Name:
Rat DAT (Dopamine Transporter) CLIA Kit
SKU:
RTES00187
Target:
Rat DAT (Dopamine Transporter)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
37.50 pg/mL
Detection range:
62.50-4000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat DAT. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat DAT and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat DAT, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat DAT. You can calculate the concentration of Rat DAT in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
92-107
87-100
97-112
Average (%)
98
93
105
1:4
Range (%)
89-103
94-107
90-102
Average (%)
95
101
96
1:8
Range (%)
103-119
95-109
91-105
Average (%)
110
102
99
1:16
Range (%)
98-113
99-117
91-104
Average (%)
104
107
98
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
99-114
105
EDTA plasma (n=5)
89-104
95
Cell culture media (n=5)
100-115
106
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
190.07
468.92
1922.87
205.93
497.77
1874.53
Standard deviation
19.33
34.79
158.06
18.08
52.51
133.47
C V (%)
10.17
7.42
8.22
8.78
10.55
7.12
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat DAT concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat DAT in samples. No significant cross-reactivity or interference between Rat DAT and analogues was observed.
