The Rat DBP (Vitamin D-binding Protein) CLIA Kit is a highly reliable and sensitive assay designed for the accurate measurement of Vitamin D-binding protein levels in rat samples such as serum, plasma, and tissue homogenates. This kit offers high specificity and reproducibility, providing researchers with consistent and trustworthy results for a variety of research applications.Vitamin D-binding protein, also known as GC protein, plays a crucial role in the transport of Vitamin D metabolites in the circulation and is involved in various physiological processes such as bone health, immune function, and inflammation.
Dysregulation of Vitamin D-binding protein levels has been implicated in various diseases including osteoporosis, chronic kidney disease, and autoimmune disorders, making it a valuable biomarker for studying these conditions and exploring potential therapeutic interventions.Overall, the Rat DBP (Vitamin D-binding Protein) CLIA Kit is a valuable tool for researchers looking to investigate the role of Vitamin D-binding protein in health and disease in rat models, providing accurate and precise measurements for their experiments.
Product Name:
Rat DBP (Vitamin D Binding Protein) CLIA Kit
SKU:
RTES00594
Target:
Rat DBP (Vitamin D Binding Protein)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
1.88 ng/mL
Detection range:
3.13-200 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat DBP. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat DBP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat DBP, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat DBP. You can calculate the concentration of Rat DBP in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
92-107
103-121
99-116
Average (%)
99
110
107
1:4
Range (%)
100-117
101-115
99-116
Average (%)
108
107
107
1:8
Range (%)
91-104
99-112
86-98
Average (%)
98
105
93
1:16
Range (%)
98-116
97-110
83-98
Average (%)
106
103
90
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
91-104
97
EDTA plasma (n=5)
96-111
101
Cell culture media (n=5)
98-111
105
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
10.78
24.97
98.82
11.71
24.43
103.12
Standard deviation
1.12
2.84
9.63
1.27
2.74
11.31
C V (%)
10.39
11.37
9.74
10.85
11.22
10.97
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat DBP concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat DBP in samples. No significant cross-reactivity or interference between Rat DBP and analogues was observed.
