The Rat DCN (Decorin) CLIA Kit is specifically designed for the precise measurement of decorin levels in rat samples, including serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, producing consistent and accurate results suitable for various research purposes.Decorin is a key protein known for its roles in regulating collagen fibrillogenesis, cell growth, and tissue repair. Its dysregulation has been implicated in various pathological conditions, including fibrosis, cancer, and inflammatory disorders.
By accurately quantifying decorin levels, researchers can gain valuable insights into the mechanisms underlying these diseases and explore potential therapeutic interventions.Order the Rat DCN (Decorin) CLIA Kit today and unlock the potential for groundbreaking discoveries in the field of biomedical research.
Product Name:
Rat DCN (Decorin) CLIA Kit
SKU:
RTES00174
Target:
Rat DCN (Decorin)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
1.88 pg/mL
Detection range:
3.13-200 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat DCN. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat DCN and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat DCN, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat DCN. You can calculate the concentration of Rat DCN in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
100-115
86-99
89-106
Average (%)
105
93
97
1:4
Range (%)
101-118
90-107
89-100
Average (%)
109
97
95
1:8
Range (%)
86-102
93-110
88-103
Average (%)
93
100
95
1:16
Range (%)
94-107
102-116
101-112
Average (%)
101
109
107
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
98-116
106
EDTA plasma (n=5)
97-109
104
Cell culture media (n=5)
92-104
98
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
11.0
24.51
79.72
10.13
25.55
78.47
Standard deviation
0.95
1.91
9.11
1.31
2.52
7.91
C V (%)
8.64
7.79
11.43
12.93
9.86
10.08
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat DCN concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat DCN in samples. No significant cross-reactivity or interference between Rat DCN and analogues was observed.
