The Rat DKK1 (Dickkopf Related Protein 1) ELISA Kit is a specialized assay designed to quantitatively measure the levels of Dickkopf Related Protein 1 in rat biological samples. Dickkopf Related Protein 1 (DKK1) is known for its regulatory role in the Wnt signaling pathway, a critical signaling cascade involved in numerous cellular processes, including cell differentiation, development, and tissue homeostasis. DKK1 acts as an antagonist of Wnt signaling, modulating the canonical Wnt pathway and influencing various biological functions. Elevated or reduced levels of DKK1 can indicate disruptions in Wnt signaling and have been associated with various diseases and conditions, such as osteoporosis, cancer, and neurodegenerative disorders. Monitoring DKK1 levels through this ELISA kit provides valuable insights into the activity of the Wnt signaling pathway and the potential involvement of DKK1 in disease pathogenesis.
The Rat DKK1 ELISA Kit offered by Assay Genie ensures precise and reliable measurement of DKK1 concentrations in biological samples. With exceptional sensitivity and specificity, this kit enables accurate quantification of DKK1, facilitating research into the roles of Wnt signaling and DKK1 in various physiological and pathological processes. Manufactured under strict quality control measures, this ELISA kit delivers robust performance and user-friendly protocols, making it an ideal choice for research studies focusing on Wnt signaling modulation and associated diseases.
Product Name:
Rat DKK1 (Dickkopf Related Protein 1) ELISA Kit
SKU:
AEES00380
Target:
Rat DKK1 (Dickkopf Related Protein 1)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.19 ng/mL
Detection range:
0.31-20 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat DKK1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat DKK1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat DKK1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat DKK1. You can calculate the concentration of Rat DKK1 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
98-113
93-108
87-102
Average (%)
105
99
94
1:4
Range (%)
90-104
81-91
82-95
Average (%)
96
85
87
1:8
Range (%)
90-101
81-95
81-94
Average (%)
96
87
87
1:16
Range (%)
87-100
84-98
85-98
Average (%)
94
91
91
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
93-109
100
EDTA plasma (n=5)
86-100
93
Cell culture media (n=5)
86-100
93
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.98
3.17
8.49
0.98
3.21
7.79
Standard deviation
0.06
0.18
0.45
0.05
0.15
0.32
C V (%)
6.12
5.68
5.3
5.1
4.67
4.11
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Rat DKK1 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat DKK1 in samples. No significant cross-reactivity or interference between Rat DKK1 and analogues was observed.
