Description
Rat ECF/CCL11 (Eosinophil Chemotactic Factor) ELISA Kit
The Rat ECF/CCL11 (Eosinophil Chemotactic Factor) ELISA Kit is a specialized assay designed for the quantitative detection of Eosinophil Chemotactic Factor, also known as CCL11, in various rat biological samples. Eosinophil Chemotactic Factor (ECF/CCL11) is a chemokine that plays a crucial role in the chemotaxis and activation of eosinophils, a type of white blood cell involved in immune responses and allergic reactions. Accurate measurement of ECF/CCL11 levels using this ELISA kit can provide valuable insights into the role of eosinophils in immune responses, inflammation, and allergic diseases.
Dysregulation of ECF/CCL11 has been implicated in various inflammatory conditions and respiratory disorders, making it an important target for research in immunology and allergy-related studies. The Rat ECF/CCL11 ELISA Kit from Assay Genie offers exceptional sensitivity and specificity, ensuring accurate and reproducible results. Manufactured under stringent quality control standards, this kit provides reliable performance, ease of use, and robust analytical data, making it a valuable tool for research investigations related to eosinophil biology and immune response modulation.
| Product Name: | Rat ECF/CCL11 (Eosinophil Chemotactic Factor) ELISA Kit |
| Product Code: | AEES00394 |
| Assay Type: | Sandwich |
| Format: | 96T |
| Assay Time: | 3.5h |
| Reactivity: | Rat |
| Detection Range: | 31.25-2000 pg/mL |
| Sensitivity: | 18.75 pg/mL |
| Sample Type & Sample Volume: | Serum, plasma and other biological fluids, 100?L |
| Specificity: | This kit recognizes Rat ECF/CCL11 in samples. No significant cross-reactivity or interference between Rat ECF/CCL11 and analogues was observed. |
| Reproducibility: | Both intra-CV and inter-CV are < 10%. |
| Application: | This ELISA kit applies to the in vitro quantitative determination of Rat ECF/CCL11 concentrations in Serum, plasma and other biological fluids. |
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat ECF/CCL11. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat ECF/CCL11 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat ECF/CCL11, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat ECF/CCL11. You can calculate the concentration of Rat ECF/CCL11 in the samples by comparing the OD of the samples to the standard curve.
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Rat ECF/CCL11 were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Rat ECF/CCL11 were tested on 3 different plates, 20 replicates in each plate.
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (pg/mL) | 99.00 | 239.30 | 696.00 | 106.20 | 258.50 | 696.80 |
| Standard deviation | 5.70 | 11.70 | 25.80 | 7.30 | 11.40 | 29.30 |
| CV (%) | 5.76 | 4.89 | 3.71 | 6.87 | 4.41 | 4.20 |
Recovery
The recovery of Rat ECF/CCL11 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 85-100 | 92 |
| EDTA plasma (n=8) | 86-97 | 91 |
| Cell culture media (n=8) | 95-106 | 101 |
| Kit Components: | An unopened kit can be stored at 2-8? for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.
|
| 1. | Add 100?L standard or sample to the wells. Incubate for 90 min at 37°C |
| 2. | Discard the liquid, immediately add 100?L Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C |
| 3. | Aspirate and wash the plate for 3 times |
| 4. | Add 100?L HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times |
| 5. | Add 90?L Substrate Reagent. Incubate for 15 min at 37°C |
| 6. | Add 50?L Stop Solution |
| 7. | Read the plate at 450nm immediately. Calculation of the results |
