The Rat ELN (Elastin) CLIA Kit is specifically designed for the precise measurement of elastin levels in rat serum, plasma, and tissue homogenates. This kit offers exceptional sensitivity and specificity, guaranteeing accurate and consistent results for various research applications.Elastin is an important structural protein that provides elasticity and resilience to tissues such as the skin, blood vessels, and lungs. Changes in elastin levels have been linked to aging, cardiovascular diseases, and conditions affecting connective tissues.
Therefore, this kit serves as a valuable tool for investigating the role of elastin in these health conditions and exploring potential therapeutic interventions. The Rat ELN CLIA Kit from Assay Genie is an essential resource for researchers studying elastin biology and its implications in disease pathogenesis and treatment strategies. Get reliable data and insightful findings with this innovative kit.
Product Name:
Rat ELN (Elastin) CLIA Kit
SKU:
RTES00004
Target:
Rat ELN (Elastin)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
75.00 pg/mL
Detection range:
125.00-8000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat ELN. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat ELN and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat ELN, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat ELN. You can calculate the concentration of Rat ELN in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
103-117
89-104
87-99
Average (%)
110
96
93
1:4
Range (%)
95-110
103-117
96-108
Average (%)
102
110
102
1:8
Range (%)
89-101
85-100
91-102
Average (%)
95
92
96
1:16
Range (%)
91-105
86-100
97-115
Average (%)
96
92
105
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
89-106
97
EDTA plasma (n=5)
85-98
91
Cell culture media (n=5)
88-101
95
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
398.4
1259.47
3935.94
405.63
1297.75
3554.1
Standard deviation
36.41
94.59
338.88
39.14
110.31
369.63
C V (%)
9.14
7.51
8.61
9.65
8.5
10.4
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat ELN concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat ELN in samples. No significant cross-reactivity or interference between Rat ELN and analogues was observed.
