Rat ENA-78 (Epithelial Neutrophil Activating Peptide 78) CLIA Kit
The Rat ENA-78 (Epithelial Neutrophil Activating Peptide-78) CLIA Kit is designed for the precise measurement of ENA-78 levels in rat serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and consistent results for a variety of research purposes.ENA-78 is a key chemokine involved in the recruitment and activation of neutrophils, playing a critical role in the inflammatory response.
Elevated levels of ENA-78 have been implicated in various inflammatory conditions, making it a valuable biomarker for investigating inflammatory diseases and potential therapeutic interventions.Overall, the Rat ENA-78 CLIA Kit provides researchers with a powerful tool for studying the role of ENA-78 in inflammatory processes and developing new strategies for treating inflammatory disorders in rats.
Product Name:
Rat ENA-78 (Epithelial Neutrophil Activating Peptide 78) CLIA Kit
SKU:
RTES00203
Target:
Rat ENA-78 (Epithelial Neutrophil Activating Peptide 78)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
4.69 pg/mL
Detection range:
7.81-500 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat CXCL5. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat CXCL5 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat CXCL5, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat CXCL5. You can calculate the concentration of Rat CXCL5 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
92-105
89-100
89-106
Average (%)
99
94
96
1:4
Range (%)
86-100
86-99
100-113
Average (%)
93
93
107
1:8
Range (%)
85-98
100-111
95-113
Average (%)
91
106
103
1:16
Range (%)
94-108
92-108
101-117
Average (%)
100
99
109
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
94-108
102
EDTA plasma (n=5)
92-106
98
Cell culture media (n=5)
99-114
105
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
26.42
66.24
204.74
27.44
62.76
207.92
Standard deviation
2.89
5.8
22.79
3.28
7.2
21.33
C V (%)
10.94
8.76
11.13
11.95
11.47
10.26
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat CXCL5 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat CXCL5 in samples. No significant cross-reactivity or interference between Rat CXCL5 and analogues was observed.
