Rat FABP3 (Fatty Acid Binding Protein 3, Muscle and Heart) CLIA Kit
The Rat FABP3 (Fatty Acid Binding Protein 3) Muscle and Heart CLIA Kit is a specialized assay kit designed for the accurate measurement of FABP3 levels in rat muscle and heart tissue samples. This kit provides high sensitivity and specificity, allowing for precise and reliable quantification of FABP3 levels in biological samples.FABP3 is a key protein involved in the transport and metabolism of fatty acids in muscle and heart tissues. It plays a crucial role in energy metabolism and the regulation of lipid homeostasis, making it a valuable marker for studying metabolic disorders, heart diseases, and muscle-related conditions.
With its advanced technology and easy-to-use protocol, the Rat FABP3 Muscle and Heart CLIA Kit is an essential tool for researchers studying the role of FABP3 in various physiological and pathological processes, and for developing potential therapeutic strategies targeting FABP3-related pathways.
Product Name:
Rat FABP3 (Fatty Acid Binding Protein 3, Muscle and Heart) CLIA Kit
SKU:
RTES00507
Target:
Rat FABP3 (Fatty Acid Binding Protein 3, Muscle and Heart)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat FABP3. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat FABP3 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat FABP3, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat FABP3. You can calculate the concentration of Rat FABP3 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
93-107
93-111
94-109
Average (%)
100
101
100
1:4
Range (%)
103-116
97-114
101-113
Average (%)
110
105
107
1:8
Range (%)
92-106
85-99
89-102
Average (%)
98
91
94
1:16
Range (%)
101-118
94-107
94-109
Average (%)
108
101
100
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
90-103
95
EDTA plasma (n=5)
84-98
91
Cell culture media (n=5)
86-97
91
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
91.61
261.81
888.57
89.03
238.87
943.11
Standard deviation
10.27
20.76
66.29
7.51
17.39
97.52
C V (%)
11.21
7.93
7.46
8.44
7.28
10.34
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat FABP3 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat FABP3 in samples. No significant cross-reactivity or interference between Rat FABP3 and analogues was observed.
