The Rat Farnesoid X Receptor (FXR) ELISA Kit is specifically designed for the precise measurement of FXR levels in rat serum, plasma, and tissue homogenates. With its exceptional sensitivity and specificity, this kit delivers highly accurate and reproducible results, making it an invaluable tool for researchers in various fields.FXR is a key nuclear receptor that plays a fundamental role in regulating bile acid homeostasis, lipid metabolism, and glucose metabolism.
Dysregulation of FXR has been linked to numerous metabolic disorders, including non-alcoholic fatty liver disease and diabetes, making it a critical target for therapeutic interventions.By utilizing the Rat FXR ELISA Kit, researchers can gain valuable insights into the role of FXR in health and disease, facilitating the development of novel treatment strategies and advancing our understanding of metabolic disorders in rats.
Product Name:
Rat Farnesoid X Receptor (FXR) ELISA Kit
Product Code:
RTDL00400
Size:
96 Assays
Target:
FXR
Synonyms:
NR1H4, BAR, FXR, HRR1, RIP14, Nuclear Receptor Subfamily 1,Group H,Member 4, Bile acid receptor, Farnesol receptor HRR-1, Retinoid X receptor-interacting protein 14
Detection Method:
Sandwich
Range:
0.156-10ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-99
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
