The Rat FXIIII Coagulation Factor XII CLIA Kit is a highly sensitive and specific tool designed for the accurate detection of Factor XII levels in rat serum, plasma, and cell culture supernatants. This kit provides reliable and reproducible results, making it ideal for a variety of research applications.Factor XII, also known as Hageman factor, is an essential component of the coagulation cascade and plays a crucial role in blood clotting.
Dysregulation of Factor XII has been linked to various bleeding disorders and thrombotic conditions, making it a key biomarker for studying these diseases and developing potential treatments.With its advanced technology and user-friendly protocol, the Rat FXIIII Coagulation Factor XII CLIA Kit is a valuable tool for researchers studying coagulation disorders and seeking to better understand the role of Factor XII in health and disease.
Product Name:
Rat FXIIII (Coagulation Factor XII) CLIA Kit
SKU:
RTES00132
Target:
Rat FXIIII (Coagulation Factor XII)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
75.00 pg/mL
Detection range:
125.00-8000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat F12. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat F12 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat F12, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat F12. You can calculate the concentration of Rat F12 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
96-107
93-108
86-96
Average (%)
101
100
91
1:4
Range (%)
99-116
94-109
98-112
Average (%)
106
102
106
1:8
Range (%)
98-109
94-106
95-109
Average (%)
104
100
102
1:16
Range (%)
96-108
104-118
88-100
Average (%)
102
110
93
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
93-107
98
EDTA plasma (n=5)
95-110
101
Cell culture media (n=5)
89-102
95
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
437.47
685.58
3146.69
422.8
721.59
3416.12
Standard deviation
46.11
63.96
305.86
39.15
54.91
312.23
C V (%)
10.54
9.33
9.72
9.26
7.61
9.14
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat F12 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat F12 in samples. No significant cross-reactivity or interference between Rat F12 and analogues was observed.
