Description
Rat G-CSF (Granulocyte Colony Stimulating Factor) ELISA Kit
The Rat G-CSF (Granulocyte Colony Stimulating Factor) ELISA Kit is a specialized assay designed to quantitatively measure G-CSF levels in various biological samples from rats. G-CSF is a crucial cytokine that promotes the production, differentiation, and activation of granulocytes, particularly neutrophils, in response to infection or injury. This cytokine plays a key role in regulating leukocyte development and mobilization, contributing to the body's immune defense mechanisms. This ELISA kit provides researchers with a precise and reliable tool to assess G-CSF concentrations, offering valuable insights into the regulation of granulopoiesis and inflammatory responses.
By accurately quantifying G-CSF levels, scientists can further explore its functions in hematopoiesis, inflammation, and immune-mediated conditions. The Rat G-CSF (Granulocyte Colony Stimulating Factor) ELISA Kit from Assay Genie ensures exceptional sensitivity and specificity, guaranteeing accurate and reproducible results. With stringent quality control measures in place, this kit delivers reliable performance and user-friendly operation, making it an ideal choice for research purposes in immunology, hematology, and related fields studying G-CSF biology in rat models.
| Product Name: | Rat G-CSF (Granulocyte Colony Stimulating Factor) ELISA Kit |
| Product Code: | AEES00418 |
| Assay Type: | Sandwich |
| Format: | 96T |
| Assay Time: | 3.5h |
| Reactivity: | Rat |
| Detection Range: | 15.63-1000 pg/mL |
| Sensitivity: | 9.38 pg/mL |
| Sample Type & Sample Volume: | Serum, plasma and other biological fluids, 100?L |
| Specificity: | This kit recognizes Rat G-CSF in samples. No significant cross-reactivity or interference between Rat G-CSF and analogues was observed. |
| Reproducibility: | Both intra-CV and inter-CV are < 10%. |
| Application: | This ELISA kit applies to the in vitro quantitative determination of Rat G-CSF concentrations in serum, plasma and other biological fluids. |
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat G-CSF. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat G-CSF and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat G-CSF, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat G-CSF. You can calculate the concentration of Rat G-CSF in the samples by comparing the OD of the samples to the standard curve.
| Kit Components: | An unopened kit can be stored at 2-8? for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.
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| 1. | Add 100?L standard or sample to the wells. Incubate for 90 min at 37°C |
| 2. | Discard the liquid, immediately add 100?L Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C |
| 3. | Aspirate and wash the plate for 3 times |
| 4. | Add 100?L HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times |
| 5. | Add 90?L Substrate Reagent. Incubate for 15 min at 37°C |
| 6. | Add 50?L Stop Solution |
| 7. | Read the plate at 450nm immediately. Calculation of the results |
