The Rat Gal3 (Galectin-3) CLIA Kit is specifically designed for the precise measurement of galectin-3 levels in rat samples, including serum, plasma, and tissue homogenates. This kit offers exceptional sensitivity and specificity, ensuring highly accurate and consistent results for various research applications.Galectin-3 is a multifunctional protein involved in various cellular processes, including inflammation, fibrosis, and cancer progression.
It is a key player in the regulation of immune responses and tissue repair, making it a valuable biomarker for studying a wide range of diseases and potential therapeutic interventions.With its advanced technology and reliable performance, the Rat Gal3 CLIA Kit is an essential tool for researchers looking to investigate the role of galectin-3 in different physiological and pathological conditions in rat models.
Product Name:
Rat GAL3 (Galectin 3) CLIA Kit
SKU:
RTES00224
Target:
Rat GAL3 (Galectin 3)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
7.50 pg/mL
Detection range:
12.50-800 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat GAL3. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat GAL3 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat GAL3, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat GAL3. You can calculate the concentration of Rat GAL3 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
103-115
92-105
96-109
Average (%)
109
98
103
1:4
Range (%)
97-113
93-107
85-97
Average (%)
104
99
90
1:8
Range (%)
85-99
100-114
95-108
Average (%)
91
108
102
1:16
Range (%)
100-113
98-114
100-113
Average (%)
106
106
105
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
100-115
107
EDTA plasma (n=5)
98-113
103
Cell culture media (n=5)
90-105
96
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
37.25
80.83
304.14
38.85
80.31
284.95
Standard deviation
4.38
8.45
28.89
3.37
8.05
30.09
C V (%)
11.76
10.45
9.5
8.67
10.02
10.56
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat GAL3 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat GAL3 in samples. No significant cross-reactivity or interference between Rat GAL3 and analogues was observed.
