Rat GFAP (Glial Fibrillary Acidic Protein) CLIA Kit
The Rat GFAP (Glial Fibrillary Acidic Protein) CLIA Kit is specifically designed for the accurate and reliable detection of GFAP levels in rat samples, including serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring precise and reproducible results for a variety of research applications in the field of neuroscience.GFAP is a key protein found in astrocytes, the most abundant type of glial cells in the central nervous system. Increased GFAP expression is associated with various neurological conditions, including brain injury, Alzheimer's disease, and multiple sclerosis.
By accurately measuring GFAP levels, researchers can gain valuable insights into the pathogenesis of these diseases and potentially identify novel therapeutic targets.Overall, the Rat GFAP CLIA Kit from Assay Genie provides researchers with a powerful tool to study the role of GFAP in neurodegenerative disorders and advance our understanding of brain health and disease.
Product Name:
Rat GFAP (Glial Fibrillary Acidic Protein) CLIA Kit
SKU:
RTES00628
Target:
Rat GFAP (Glial Fibrillary Acidic Protein)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
37.50 pg/mL
Detection range:
62.50-4000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat GFAP. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat GFAP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat GFAP, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat GFAP. You can calculate the concentration of Rat GFAP in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
93-107
94-107
90-105
Average (%)
100
100
96
1:4
Range (%)
101-118
86-97
87-100
Average (%)
108
92
94
1:8
Range (%)
98-112
92-107
89-102
Average (%)
106
98
96
1:16
Range (%)
101-114
94-106
89-101
Average (%)
107
101
95
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
90-107
97
EDTA plasma (n=5)
84-100
91
Cell culture media (n=5)
101-117
108
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
198.82
517.36
1456.27
214.62
541.21
1491.35
Standard deviation
16.22
43.46
156.26
26.27
59.7
141.68
C V (%)
8.16
8.4
10.73
12.24
11.03
9.5
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat GFAP concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat GFAP in samples. No significant cross-reactivity or interference between Rat GFAP and analogues was observed.
