The Rat GH (Growth Hormone) CLIA Kit is a highly reliable and accurate tool for detecting levels of growth hormone in rat serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit delivers consistent and reproducible results, making it suitable for various research applications.Growth hormone is a vital hormone that plays a crucial role in regulating growth, metabolism, and overall development in rats. Dysfunction in growth hormone levels can lead to various health issues, making it essential to accurately measure and monitor these levels in research studies.
Whether studying growth disorders, metabolic diseases, or the effects of various treatments on growth hormone levels, the Rat GH CLIA Kit provides researchers with a valuable tool for conducting thorough and accurate analyses. Trust in the precision and reliability of this kit to support your research efforts effectively.
Product Name:
Rat GH (Growth Hormone) CLIA Kit
SKU:
RTES00022
Target:
Rat GH (Growth Hormone)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
37.50 pg/mL
Detection range:
62.50-4000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat GH. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat GH and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat GH, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat GH. You can calculate the concentration of Rat GH in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
97-114
96-110
89-103
Average (%)
104
104
94
1:4
Range (%)
91-108
97-113
102-118
Average (%)
98
105
109
1:8
Range (%)
93-109
92-103
99-114
Average (%)
101
97
106
1:16
Range (%)
100-116
101-116
89-100
Average (%)
107
107
95
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
95-112
102
EDTA plasma (n=5)
90-104
97
Cell culture media (n=5)
91-104
98
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
203.42
442.54
1413.65
197.39
420.54
1514.94
Standard deviation
20.28
33.72
151.12
18.36
38.35
138.01
C V (%)
9.97
7.62
10.69
9.3
9.12
9.11
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat GH concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat GH in samples. No significant cross-reactivity or interference between Rat GH and analogues was observed.
