The Rat Ghrelin ELISA Kit is specifically designed for the precise measurement of ghrelin levels in rat serum, plasma, and tissue homogenates. This kit offers exceptional sensitivity and specificity, guaranteeing trustworthy and consistent results for various research purposes. Ghrelin is a vital hormone known to regulate appetite, energy metabolism, and growth hormone release. It plays a crucial role in conditions such as obesity, diabetes, and gastrointestinal disorders, making it a valuable biomarker for studying these diseases and exploring therapeutic interventions.
Overall, the Rat Ghrelin ELISA Kit is a valuable tool for researchers seeking to investigate the role of ghrelin in physiological processes and disease states, providing accurate and reliable data for further analysis and interpretation.
Product Name:
Rat GHRL (Ghrelin) ELISA Kit
SKU:
RTES00707
Target:
Rat GHRL (Ghrelin)
Size:
96T
Assay type:
Competitive-ELISA
Assay time:
2.0h
Sensitivity:
0.10 ng/mL
Detection range:
0.16-10 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with GHRL. During the reaction, GHRL in the sample or standard competes with a fixed amount of GHRL on the solid phase supporter for sites on the Biotinylated Detection Ab specific to GHRL. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of GHRL in tested samples can be calculated by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
86-99
88-100
96-111
Average (%)
91
93
104
1:4
Range (%)
86-98
93-107
95-112
Average (%)
93
98
102
1:8
Range (%)
90-103
91-104
93-105
Average (%)
95
97
100
1:16
Range (%)
91-103
89-100
97-111
Average (%)
96
95
105
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
92-103
97
EDTA plasma (n=5)
85-101
92
Cell culture media (n=5)
93-106
98
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.5
0.9
3.8
0.5
0.9
4.0
Standard deviation
0.03
0.05
0.13
0.03
0.04
0.2
C V (%)
6
5.56
3.42
6.0
4.44
5.0
Sample type &Sample volume:
serum, plasma and other biological fluids; 50μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of GHRL concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes GHRL in samples. No significant cross-reactivity or interference between GHRL and analogues was observed.
