Rat GM-CSF (Granulocyte-Macrophage Colony Stimulating Factor) CLIA Kit
The Rat GM-CSF (Granulocyte-Macrophage Colony-Stimulating Factor) CLIA Kit is a highly sensitive and specific assay for the accurate detection of GM-CSF levels in rat serum, plasma, and cell culture supernatants. This innovative kit provides reliable and reproducible results, making it ideal for a variety of research applications.GM-CSF is a critical cytokine involved in the regulation of immune cell development and function, including the stimulation of granulocyte and macrophage production.
Dysregulation of GM-CSF has been implicated in various diseases such as inflammatory disorders and autoimmune conditions, highlighting its importance as a biomarker for disease progression and therapeutic development.The Rat GM-CSF CLIA Kit from Assay Genie offers researchers a valuable tool for investigating the role of GM-CSF in various physiological and pathological processes, providing crucial insights for advancing our understanding of immune regulation and disease mechanisms in rats.
Product Name:
Rat GM-CSF (Granulocyte-Macrophage Colony Stimulating Factor) CLIA Kit
SKU:
RTES00008
Target:
Rat GM-CSF (Granulocyte-Macrophage Colony Stimulating Factor)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
4.69 pg/mL
Detection range:
7.81-500 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat GM-CSF. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat GM-CSF and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat GM-CSF, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat GM-CSF. You can calculate the concentration of Rat GM-CSF in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
93-106
91-105
87-103
Average (%)
100
97
94
1:4
Range (%)
90-101
99-115
99-115
Average (%)
95
107
106
1:8
Range (%)
95-108
87-101
97-113
Average (%)
101
93
104
1:16
Range (%)
86-100
90-106
104-116
Average (%)
92
97
110
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
92-104
98
EDTA plasma (n=5)
99-111
105
Cell culture media (n=5)
96-111
102
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
25.3
44.77
175.19
24.67
40.32
160.42
Standard deviation
2.17
4.18
14.24
2.14
4.62
12.27
C V (%)
8.58
9.34
8.13
8.67
11.46
7.65
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat GM-CSF concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat GM-CSF in samples. No significant cross-reactivity or interference between Rat GM-CSF and analogues was observed.
