The Rat GP130 (Glucoprotein 130) CLIA Kit is a highly sensitive and specific assay designed for the accurate detection of GP130 levels in rat serum, plasma, and cell culture supernatants. This kit provides reliable and reproducible results, making it ideal for a variety of research applications.GP130 is a critical protein that plays a key role in signaling pathways involved in inflammation, immune response, and cell growth. Dysregulation of GP130 has been linked to various diseases, including cancer, autoimmune disorders, and metabolic conditions.
As such, measuring GP130 levels can provide valuable insights into disease progression and potential therapeutic interventions.With its user-friendly protocol and high-performance capabilities, the Rat GP130 CLIA Kit from Assay Genie is an essential tool for researchers studying the role of GP130 in health and disease. Get accurate and reliable results with this cutting-edge assay kit.
Product Name:
Rat gp130 (Glucoprotein 130) CLIA Kit
SKU:
RTES00243
Target:
Rat gp130 (Glucoprotein 130)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat gp130. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat gp130 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat gp130, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat gp130. You can calculate the concentration of Rat gp130 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
85-97
92-106
101-119
Average (%)
91
97
108
1:4
Range (%)
84-95
88-100
91-106
Average (%)
90
95
97
1:8
Range (%)
90-101
98-115
101-117
Average (%)
95
105
108
1:16
Range (%)
97-112
93-108
91-103
Average (%)
104
99
96
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
88-101
95
EDTA plasma (n=5)
99-112
106
Cell culture media (n=5)
102-114
107
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
96.39
167.15
827.9
90.02
165.98
851.92
Standard deviation
11.59
13.14
80.72
8.26
16.5
82.55
C V (%)
12.02
7.86
9.75
9.18
9.94
9.69
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat gp130 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat gp130 in samples. No significant cross-reactivity or interference between Rat gp130 and analogues was observed.
