The Rat GPX1 (Glutathione Peroxidase 1) ELISA Kit is designed for the accurate quantification of GPX1 levels in rat serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring reliable and reproducible results for a variety of research applications.GPX1 is an important antioxidant enzyme that plays a key role in protecting cells from oxidative damage by reducing hydrogen peroxide and lipid peroxides. Dysregulation of GPX1 has been implicated in various diseases such as cancer, inflammatory conditions, and neurodegenerative disorders, making it a valuable biomarker for investigating these pathologies and potential therapeutic interventions.
By utilizing the Rat GPX1 ELISA Kit, researchers can gain valuable insights into the role of GPX1 in health and disease, leading to a better understanding of cellular oxidative stress and potential treatment strategies.
Product Name:
Rat GPX1 (Glutathione Peroxidase 1) ELISA Kit
SKU:
RTES01107
Target:
Rat GPX1 (Glutathione Peroxidase 1)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat GPX1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat GPX1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat GPX1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat GPX1. You can calculate the concentration of Rat GPX1 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
95-108
87-103
94-107
Average (%)
101
94
101
1:4
Range (%)
92-108
83-96
85-99
Average (%)
99
90
91
1:8
Range (%)
92-104
85-98
87-101
Average (%)
97
91
93
1:16
Range (%)
92-106
81-93
88-100
Average (%)
99
88
95
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
92-107
99
EDTA plasma (n=5)
91-105
97
Cell culture media (n=5)
86-100
92
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
96.24
211.75
917.74
87.24
225.09
838.83
Standard deviation
6.06
11.96
27.81
4.72
11.77
29.95
C V (%)
6.3
5.65
3.03
5.41
5.23
3.57
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Rat GPX1 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat GPX1 in samples. No significant cross-reactivity or interference between Rat GPX1 and analogues was observed.
