Rat GRO alpha/CXCL1 (Growth Regulated Oncogene Alpha) CLIA Kit
The Rat GRO-alpha/CXCL1 CLIA Kit is a powerful tool for analyzing levels of the Growth-Regulated Oncogene-Alpha (GRO-alpha) in rat samples. GRO-alpha is a key chemokine involved in inflammation and immune response, making it a valuable marker for studying inflammatory diseases, cancer, and other conditions.This kit offers exceptional sensitivity and accuracy, ensuring precise and reliable results for your research needs. Whether working with serum, plasma, or cell culture supernatants, this kit provides the performance and versatility necessary for a wide range of experimental applications.
By accurately measuring GRO-alpha levels, researchers can gain valuable insights into the mechanisms underlying various diseases and potentially identify new therapeutic targets. Trust the Rat GRO-alpha/CXCL1 CLIA Kit to deliver the data you need to advance your research and uncover novel discoveries in the field of inflammation and immunology.
Product Name:
Rat GRO alpha/CXCL1 (Growth Regulated Oncogene Alpha) CLIA Kit
SKU:
RTES00003
Target:
Rat GRO alpha/CXCL1 (Growth Regulated Oncogene Alpha)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
4.69 pg/mL
Detection range:
7.81-500 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat GROα/CXCL1. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat GROα/CXCL1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat GROα/CXCL1, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat GROα/CXCL1. You can calculate the concentration of Rat GROα/CXCL1 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
99-115
89-105
101-114
Average (%)
107
96
107
1:4
Range (%)
100-112
90-106
97-112
Average (%)
106
97
104
1:8
Range (%)
91-104
85-96
98-115
Average (%)
99
90
106
1:16
Range (%)
98-114
85-98
92-103
Average (%)
105
91
98
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
84-99
90
EDTA plasma (n=5)
92-106
99
Cell culture media (n=5)
96-110
103
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
22.79
65.44
213.3
21.16
64.47
209.54
Standard deviation
1.98
6.4
18.71
2.64
7.49
23.76
C V (%)
8.69
9.78
8.77
12.48
11.62
11.34
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat GROα/CXCL1 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat GROα/CXCL1 in samples. No significant cross-reactivity or interference between Rat GROα/CXCL1 and analogues was observed.
