The Rat GT-34 Gastrin-34 CLIA Kit is a cutting-edge assay designed for the accurate and reliable detection of GT-34 Gastrin-34 levels in rat samples such as serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and reproducible results, making it an invaluable tool for a wide array of research applications. GT-34 Gastrin-34 is a key peptide hormone involved in regulating gastric acid secretion, stomach motility, and gastrointestinal functions.
Imbalances in GT-34 Gastrin-34 levels have been linked to various gastrointestinal disorders, making this kit an essential resource for studying these conditions and potential therapeutic interventions.Overall, the Rat GT-34 Gastrin-34 CLIA Kit provides researchers with a powerful tool to explore the role of GT-34 Gastrin-34 in physiological and pathophysiological processes in rats, leading to a deeper understanding of gastrointestinal health and disease.
Product Name:
Rat GT-34 (Gastrin 34) CLIA Kit
SKU:
RTES00230
Target:
Rat GT-34 (Gastrin 34)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat GT-34. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat GT-34 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat GT-34, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat GT-34. You can calculate the concentration of Rat GT-34 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
92-107
95-108
97-115
Average (%)
98
103
105
1:4
Range (%)
101-116
98-114
90-105
Average (%)
107
106
97
1:8
Range (%)
101-116
101-118
85-100
Average (%)
109
108
91
1:16
Range (%)
86-98
92-107
91-105
Average (%)
91
99
99
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
90-104
96
EDTA plasma (n=5)
89-101
94
Cell culture media (n=5)
89-105
96
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
46.7
158.92
477.35
50.72
157.08
521.27
Standard deviation
4.49
14.3
41.62
5.44
18.03
48.63
C V (%)
9.61
9.0
8.72
10.73
11.48
9.33
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat GT-34 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat GT-34 in samples. No significant cross-reactivity or interference between Rat GT-34 and analogues was observed.
