Description
Rat HABP1/C1QBP (Hyaluronan Binding Protein 1) ELISA Kit
The Rat HABP1/C1QBP (Hyaluronan Binding Protein 1) ELISA Kit is a sophisticated assay designed for the accurate quantification of Hyaluronan Binding Protein 1 levels in various biological samples. Hyaluronan Binding Protein 1, also known as C1QBP, is a multifunctional protein involved in diverse cellular processes, including hyaluronan binding, complement activation, and regulation of mitochondrial function.
This protein plays a crucial role in cellular homeostasis and is implicated in various physiological and pathological conditions. Accurate measurement of Hyaluronan Binding Protein 1 is essential for understanding its role in cell-matrix interactions, immune responses, and mitochondrial activities. The Rat HABP1/C1QBP ELISA Kit provided by Assay Genie ensures exceptional sensitivity and specificity, allowing for reliable and reproducible results. With stringent quality control measures and user-friendly protocols, this kit delivers robust performance, making it an indispensable tool for cutting-edge research in biological and medical sciences.
| Product Name: | Rat HABP1/C1QBP (Hyaluronan Binding Protein 1) ELISA Kit |
| Product Code: | AEES00437 |
| Assay Type: | Sandwich |
| Format: | 96T |
| Assay Time: | 3.5h |
| Reactivity: | Rat |
| Detection Range: | 0.31-20 ng/mL |
| Sensitivity: | 0.19 ng/mL |
| Sample Type & Sample Volume: | Serum, plasma and other biological fluids, 100?L |
| Specificity: | This kit recognizes Rat HABP1/C1QBP in samples. No significant cross-reactivity or interference between Rat HABP1/C1QBP and analogues was observed. |
| Reproducibility: | Both intra-CV and inter-CV are < 10%. |
| Application: | This ELISA kit applies to the in vitro quantitative determination of Rat HABP1/C1QBP concentrations in serum, plasma and other biological fluids. |
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat HABP1/C1QBP. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat HABP1/C1QBP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat HABP1/C1QBP, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat HABP1/C1QBP. You can calculate the concentration of Rat HABP1/C1QBP in the samples by comparing the OD of the samples to the standard curve.
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Rat HABP1/C1QBP were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Rat HABP1/C1QBP were tested on 3 different plates, 20 replicates in each plate.
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (ng/mL) | 0.93 | 1.94 | 9.87 | 1.00 | 2.00 | 10.06 |
| Standard deviation | 0.05 | 0.11 | 0.42 | 0.07 | 0.10 | 0.39 |
| CV (%) | 5.38 | 5.67 | 4.26 | 7.00 | 5.00 | 3.88 |
Recovery
The recovery of Rat HABP1/C1QBP spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 90-104 | 97 |
| EDTA plasma (n=8) | 90-105 | 96 |
| Cell culture media (n=8) | 88-98 | 93 |
| Kit Components: | An unopened kit can be stored at 2-8? for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.
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| 1. | Add 100?L standard or sample to the wells. Incubate for 90 min at 37°C |
| 2. | Discard the liquid, immediately add 100?L Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C |
| 3. | Aspirate and wash the plate for 3 times |
| 4. | Add 100?L HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times |
| 5. | Add 90?L Substrate Reagent. Incubate for 15 min at 37°C |
| 6. | Add 50?L Stop Solution |
| 7. | Read the plate at 450nm immediately. Calculation of the results |
