The Rat Hedgehog Homolog Indian (IHH) ELISA Kit is specifically designed for the precise measurement of IHH levels in rat serum, plasma, and tissue homogenates. This kit offers exceptional sensitivity and specificity, guaranteeing accurate and consistent results for various research purposes.IHH is a key signaling molecule that plays a critical role in embryonic development, particularly in the patterning of various tissues and organs. Dysregulation of IHH signaling has been implicated in a variety of pathological conditions such as developmental disorders, skeletal abnormalities, and certain types of cancer.
Therefore, detecting and quantifying IHH levels can provide valuable insights into these diseases and aid in the development of potential therapeutic interventions.This cutting-edge ELISA kit from Assay Genie enables researchers to effectively study the role of IHH in disease pathology and can serve as a valuable tool in advancing biomedical research and drug discovery efforts. Trust in the accuracy and reliability of the Rat Hedgehog Homolog Indian (IHH) ELISA Kit for your experimental needs.
Product Name:
Rat Hedgehog Homolog, Indian (IHH) ELISA Kit
Product Code:
RTDL00535
Size:
96 Assays
Target:
IHH
Synonyms:
BDA1, HHG2
Detection Method:
Sandwich
Range:
0.156-10ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-99
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
