The Rat Hepcidin CLIA Kit (RTES00295) from AssayGenie is a highly sensitive and specific assay designed for the accurate detection of hepcidin levels in rat samples. This kit is ideal for researchers studying iron metabolism, anemia, and inflammatory conditions where hepcidin plays a crucial role.Hepcidin is a key regulator of iron homeostasis, controlling iron absorption and distribution in the body. Dysregulation of hepcidin levels has been linked to various health conditions, including iron overload disorders and anemia.
By accurately measuring hepcidin levels in rat samples, researchers can gain valuable insights into the mechanisms underlying these diseases and develop potential therapeutic strategies.With its reliable performance and ease of use, the Rat Hepcidin CLIA Kit from AssayGenie is a valuable tool for advancing research in the field of iron metabolism and related disorders. Order yours today and unlock new discoveries in your scientific investigations.
Product Name:
Rat Hepc (Hepcidin) CLIA Kit
SKU:
RTES00295
Target:
Rat Hepc (Hepcidin)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat Hepc. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat Hepc and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat Hepc, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat Hepc. You can calculate the concentration of Rat Hepc in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
85-99
99-113
89-100
Average (%)
91
106
94
1:4
Range (%)
88-100
96-108
90-103
Average (%)
93
102
96
1:8
Range (%)
94-109
97-113
86-101
Average (%)
101
105
93
1:16
Range (%)
103-116
99-115
93-107
Average (%)
109
106
100
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
93-107
100
EDTA plasma (n=5)
85-98
91
Cell culture media (n=5)
99-115
106
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
50.7
154.32
434.25
55.25
153.75
417.35
Standard deviation
6.5
17.25
33.31
4.92
13.82
31.13
C V (%)
12.82
11.18
7.67
8.9
8.99
7.46
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat Hepc concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat Hepc in samples. No significant cross-reactivity or interference between Rat Hepc and analogues was observed.
