The Rat Hepc (Hepcidin) ELISA Kit is expertly designed to accurately quantify Hepcidin levels in diverse rat biological samples. Hepcidin is a vital peptide hormone that plays a crucial role in iron regulation within the body. It is primarily synthesized in the liver and acts as a key regulator of iron metabolism, controlling iron absorption in the intestine, iron release from macrophages, and iron entry into circulation. By measuring Hepcidin levels, researchers can gain valuable insights into iron homeostasis, erythropoiesis, and the pathophysiology of iron-related disorders. Accurate quantification of Hepcidin is essential for unraveling its intricate role in iron balance and its implications in various health conditions. The Rat Hepc ELISA Kit offers outstanding sensitivity and specificity, ensuring precise and reproducible results critical for advancing iron metabolism research. Manufactifiable to stringent quality control standards, this kit delivers robust performance and user-friendly protocols, making it an excellent tool for researchers investigating the functions of Hepcidin in iron regulation dynamics and related disorders.
Product Name:
Rat Hepc (Hepcidin) ELISA Kit
SKU:
AEES00432
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
46.88 pg/mL
Detection range:
78.13-5000 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
