The Rat HGF (Hepatocyte Growth Factor) CLIA Kit is specifically designed for the quantitative detection of HGF levels in rat serum, plasma, and tissue homogenates. This highly sensitive and specific kit provides accurate and reproducible results, making it ideal for various research applications.HGF is a key growth factor involved in liver regeneration, wound healing, and tissue repair. It plays a critical role in promoting cell growth, migration, and survival, making it a valuable biomarker for studying liver diseases, fibrosis, and tissue regeneration.
By using the Rat HGF CLIA Kit, researchers can effectively measure HGF levels in biological samples, allowing for a better understanding of its role in various physiological and pathological processes. This kit is a valuable tool for advancing research in liver biology, regenerative medicine, and disease modeling.
Product Name:
Rat HGF (Hepatocyte Growth Factor) CLIA Kit
SKU:
RTES00292
Target:
Rat HGF (Hepatocyte Growth Factor)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat HGF. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat HGF and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat HGF, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat HGF. You can calculate the concentration of Rat HGF in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
88-102
86-100
96-107
Average (%)
94
93
102
1:4
Range (%)
99-117
104-119
100-117
Average (%)
106
110
108
1:8
Range (%)
91-105
87-100
91-105
Average (%)
97
93
97
1:16
Range (%)
91-104
98-112
98-115
Average (%)
97
103
106
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
99-112
106
EDTA plasma (n=5)
88-100
93
Cell culture media (n=5)
95-110
103
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
46.26
134.3
346.2
49.13
135.85
334.22
Standard deviation
4.23
14.41
24.65
6.24
12.97
23.23
C V (%)
9.14
10.73
7.12
12.7
9.55
6.95
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat HGF concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat HGF in samples. No significant cross-reactivity or interference between Rat HGF and analogues was observed.
