Rat High Affinity Choline Transporter (ChT) ELISA Kit
The Rat High Affinity Choline Transporter (CHT) ELISA Kit is a valuable tool for accurately measuring levels of the high affinity choline transporter in rat samples including serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring precise and reliable results for various research experiments.The high affinity choline transporter is a key component in choline uptake and acetylcholine synthesis, playing a critical role in neurotransmission and cognitive function. Dysregulation of this transporter has been implicated in neurological disorders such as Alzheimer's disease and schizophrenia, making it an important target for further investigation and potential therapeutic interventions.
Overall, the Rat High Affinity Choline Transporter ELISA Kit is an essential resource for researchers studying choline metabolism, neurotransmission, and neurodegenerative diseases in rat models. Its accuracy and precision make it an indispensable tool for advancing our understanding of choline transport mechanisms and their implications in health and disease.
Product Name:
Rat High Affinity Choline Transporter (ChT) ELISA Kit
Product Code:
RTDL00213
Size:
96 Assays
Target:
ChT
Synonyms:
SLC5A7, hCHT, CHT1, Solute Carrier Family 5 Member 7,Choline Transporter, Hemicholinium-3-sensitive choline transporter
Detection Method:
Sandwich
Range:
78.125-5000pg/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
85-101
93
EDTA plasma (n=5)
86-98
92
Heparin plasma (n=5)
87-101
94
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
