Rat High Mobility Group Protein 1 (HMG1) ELISA Kit
The Rat High Mobility Group Protein 1 (HMG1) ELISA Kit is a powerful tool for the quantification of HMG1 levels in rat serum, plasma, and tissue homogenates. This kit offers exceptional sensitivity and specificity, ensuring precise and reliable results for a variety of research applications.High Mobility Group Protein 1 (HMG1) is a key regulator of inflammation and immune response, playing a critical role in various diseases such as sepsis, autoimmune disorders, and cancer.
By accurately measuring HMG1 levels, researchers can gain valuable insights into the pathogenesis of these conditions and potential therapeutic targets.With easy-to-follow protocols and comprehensive technical support, the Rat HMG1 ELISA Kit from Assay Genie is a valuable tool for scientists studying the role of HMG1 in health and disease. Order yours today and unlock new possibilities in your research.
Product Name:
Rat High Mobility Group Protein 1 (HMG1) ELISA Kit
Product Code:
RTDL00488
Size:
96 Assays
Target:
HMG1
Synonyms:
MGB1, HMG3, SBP1, Sulfoglucuronyl Carbohydrate Binding Protein, Amphoterin, High Mobility Group Box 1 Protein
Detection Method:
Sandwich
Range:
31.25-2,000pg/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
