The Rat HO-1 (Heme Oxygenase-1) CLIA Kit is a highly sensitive assay designed for the accurate measurement of HO-1 levels in rat samples such as serum, plasma, and cell culture supernatants. This kit offers superior specificity and reproducibility, making it an excellent tool for various research applications.HO-1 is an important enzyme involved in heme catabolism, playing a critical role in various biological processes such as antioxidant defense, anti-inflammatory responses, and cellular homeostasis. Dysregulation of HO-1 has been linked to several diseases including cardiovascular disorders, kidney injury, and neurodegenerative conditions, highlighting its significance as a potential biomarker for disease progression and therapeutic interventions.
With its high sensitivity and reliable performance, the Rat HO-1 CLIA Kit is an invaluable resource for researchers studying the physiological and pathological roles of HO-1 in rodent models, offering accurate and dependable results for advanced biomedical research.
Product Name:
Rat HO-1 (Heme Oxygenase 1) CLIA Kit
SKU:
RTES00284
Target:
Rat HO-1 (Heme Oxygenase 1)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat HO1. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat HO1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat HO1, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat HO1. You can calculate the concentration of Rat HO1 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
97-109
101-116
99-111
Average (%)
102
107
104
1:4
Range (%)
99-113
86-97
87-102
Average (%)
107
92
93
1:8
Range (%)
97-111
95-109
85-98
Average (%)
103
100
92
1:16
Range (%)
88-99
92-103
89-101
Average (%)
94
98
94
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
87-100
94
EDTA plasma (n=5)
100-112
106
Cell culture media (n=5)
84-97
91
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
103.92
212.77
683.28
100.13
194.89
751.37
Standard deviation
9.81
18.6
44.89
12.94
14.99
81.0
C V (%)
9.44
8.74
6.57
12.92
7.69
10.78
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat HO1 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat HO1 in samples. No significant cross-reactivity or interference between Rat HO1 and analogues was observed.
