The Rat HSF1 (Heat Shock Factor 1) CLIA Kit is specifically designed for the accurate measurement of heat shock factor 1 levels in rat samples, including serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring dependable and reproducible results for various research applications.Heat Shock Factor 1 is a key regulatory protein involved in the cellular response to stress, including heat shock and other environmental factors.
It plays a critical role in maintaining cellular homeostasis and protecting cells from damage. Dysregulation of HSF1 has been linked to various diseases, making it a valuable biomarker for studying and potentially treating these conditions.Overall, the Rat HSF1 CLIA Kit is an essential tool for researchers exploring the role of heat shock factor 1 in health and disease, providing accurate and reliable measurement of HSF1 levels in rat samples.
Product Name:
Rat HSF1 (Heat Shock Factor 1) CLIA Kit
SKU:
RTES00275
Target:
Rat HSF1 (Heat Shock Factor 1)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
37.50 pg/mL
Detection range:
62.50-4000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat HSF1. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat HSF1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat HSF1, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat HSF1. You can calculate the concentration of Rat HSF1 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
85-101
102-118
89-101
Average (%)
92
109
95
1:4
Range (%)
85-97
87-98
101-116
Average (%)
90
93
107
1:8
Range (%)
98-110
91-105
90-101
Average (%)
103
97
95
1:16
Range (%)
95-108
89-100
102-115
Average (%)
100
95
109
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
99-111
106
EDTA plasma (n=5)
89-101
95
Cell culture media (n=5)
92-103
97
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
185.95
460.56
1515.1
194.83
416.02
1568.95
Standard deviation
16.66
53.75
109.69
22.17
32.03
130.54
C V (%)
8.96
11.67
7.24
11.38
7.7
8.32
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat HSF1 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat HSF1 in samples. No significant cross-reactivity or interference between Rat HSF1 and analogues was observed.
