The Rat HSP-90 (Heat Shock Protein 90) CLIA Kit is specially designed to accurately measure levels of HSP-90 in rat samples such as serum, plasma, and cell culture supernatants. This kit provides exceptional sensitivity and specificity, ensuring precise and consistent results for various research applications.HSP-90 is a vital molecular chaperone protein that plays a crucial role in maintaining cellular homeostasis and protein folding, particularly during times of cellular stress.
Dysregulation of HSP-90 has been implicated in various diseases, including cancer, neurodegenerative disorders, and autoimmune conditions, making it a valuable biomarker for understanding disease mechanisms and potential therapeutic strategies.The Rat HSP-90 CLIA Kit offers researchers a powerful tool for studying the function and regulation of HSP-90 in rat models, ultimately advancing our understanding of its role in health and disease.
Product Name:
Rat HSP-90 (Heat Shock Protein 90) CLIA Kit
SKU:
RTES00280
Target:
Rat HSP-90 (Heat Shock Protein 90)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat HSP-90. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat HSP-90 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat HSP-90, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat HSP-90. You can calculate the concentration of Rat HSP-90 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
86-102
95-108
91-108
Average (%)
93
101
98
1:4
Range (%)
94-110
90-105
93-106
Average (%)
102
98
98
1:8
Range (%)
94-111
96-112
99-110
Average (%)
101
103
104
1:16
Range (%)
87-100
101-115
88-100
Average (%)
94
107
94
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
85-97
90
EDTA plasma (n=5)
96-110
102
Cell culture media (n=5)
98-110
104
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
97.84
171.97
837.39
105.44
163.68
900.9
Standard deviation
8.9
17.4
53.68
12.47
16.97
93.51
C V (%)
9.1
10.12
6.41
11.83
10.37
10.38
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat HSP-90 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat HSP-90 in samples. No significant cross-reactivity or interference between Rat HSP-90 and analogues was observed.
