The Rat I-PTH (Intact Parathormone) ELISA Kit is a specialized assay designed for the quantitative measurement of Intact Parathormone levels in a variety of biological samples.
Intact Parathormone, a crucial hormone produced by the parathyroid glands, plays a significant role in calcium and phosphorus homeostasis, bone metabolism, and renal function regulation. Accurate quantification of I-PTH is essential for understanding its pivotal role in maintaining mineral balance and bone health. This ELISA kit offers exceptional sensitivity and specificity, ensuring precise and reproducible results. With stringent quality control measures in place, this kit delivers reliable performance and user-friendly operation, making it a valuable tool for in-depth research applications.
Product Name:
Rat I-PTH (Intact Parathormone) ELISA Kit
SKU:
AEES00445
Target:
Rat I-PTH (Intact Parathormone)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat I-PTH. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat I-PTH and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat I-PTH, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat I-PTH. You can calculate the concentration of Rat I-PTH in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
99-113
93-107
85-99
Average (%)
105
99
91
1:4
Range (%)
90-100
84-97
84-95
Average (%)
95
91
90
1:8
Range (%)
88-104
82-95
87-98
Average (%)
95
89
92
1:16
Range (%)
89-102
79-92
84-99
Average (%)
94
85
91
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
88-102
95
EDTA plasma (n=5)
92-107
98
Cell culture media (n=5)
94-109
101
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
49.45
154.88
373.68
45.17
166.01
402.93
Standard deviation
2.6
6.91
19.51
3.04
7.29
13.94
C V (%)
5.26
4.46
5.22
6.73
4.39
3.46
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Rat I-PTH concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat I-PTH in samples. No significant cross-reactivity or interference between Rat I-PTH and analogues was observed.
