Rat IFABP/FABP2 (Intestinal Fatty Acid Binding Protein) CLIA Kit
The Rat IFABP (FABP2) Intestinal Fatty Acid Binding Protein CLIA Kit is a powerful tool for measuring levels of intestinal fatty acid binding protein in rat samples. This kit is specifically designed for use in rat serum, plasma, and tissue homogenates, offering high sensitivity and accuracy for reliable and consistent results.Intestinal fatty acid binding protein (IFABP), also known as FABP2, plays a critical role in lipid metabolism and transport in the intestine. Abnormal levels of IFABP have been linked to various gastrointestinal disorders and metabolic conditions, making it a valuable biomarker for studying these diseases and developing targeted treatments.
Whether you are conducting basic research, drug development, or clinical studies, the Rat IFABP (FABP2) Intestinal Fatty Acid Binding Protein CLIA Kit provides a comprehensive solution for analyzing IFABP levels in rat samples with ease and precision. Trust in this kit to enhance your research and advance your understanding of intestinal fatty acid binding protein biology.
Product Name:
Rat IFABP/FABP2 (Intestinal Fatty Acid Binding Protein) CLIA Kit
SKU:
RTES00350
Target:
Rat IFABP/FABP2 (Intestinal Fatty Acid Binding Protein)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat IFABP/FABP2. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat IFABP/FABP2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat IFABP/FABP2, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat IFABP/FABP2. You can calculate the concentration of Rat IFABP/FABP2 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
89-103
103-117
91-106
Average (%)
95
110
98
1:4
Range (%)
102-118
100-113
85-99
Average (%)
110
107
92
1:8
Range (%)
101-117
102-116
100-115
Average (%)
109
109
107
1:16
Range (%)
101-117
102-117
91-105
Average (%)
107
109
97
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
96-109
101
EDTA plasma (n=5)
89-103
96
Cell culture media (n=5)
88-103
95
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
108.42
280.81
701.2
103.12
270.05
756.64
Standard deviation
10.46
22.35
44.53
8.72
29.33
64.24
C V (%)
9.65
7.96
6.35
8.46
10.86
8.49
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat IFABP/FABP2 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat IFABP/FABP2 in samples. No significant cross-reactivity or interference between Rat IFABP/FABP2 and analogues was observed.
